BISC 520 Medical Microbiology


Write a report on the topic Bacterial Clonal Diagnostics as an Evidence-Based Emiric Antibiotic Selection Tool



Because there is no way to know if you are susceptible, antimicrobial resistance has become a significant problem for healthcare systems.

Therefore, it is important to use different speed approaches to determine susceptibility.

Researchers evaluated the efficacy of the empirical antibiotic selection clonal diagnostic approach in this paper.

This paper uses an observational cohort and quantitative PCR tests to determine 50 Escherichia Coli clonal groups. They also significantly reduced the likelihood of antibiotic mismatch.


For the determination of antibiotic susceptibility or cloning, there were several methods. These included prospective clonal diagnosis study, 7SNP test, local and multinational reference sets, urine culture and isolation testing, statistical analysis, as well as calculations related to prescription mismatch rate.

Data derived from various tests were used for statistical analysis.

In addition, the method 20, 10, and 30 resistance thresholds were allowed to be determined.


The 7-SNP testing revealed that 274 of the 750 urine samples contained E.coli.

There were 267 E.coli cases among the samples. The culture results showed that 260 of them (97%) had the same clonotype as single-colony testing.

The prevalence of resistance against FQs was 21% higher than that of 15%, P =.004, and CZ (8 vs. 15%) was lower (P =.001) and NIT (0.4vs. 5%; P.001).

With respect to antibiotic therapy options, the 7-SNP testing would allow FQs for 78.6%. T/S for 57%. CZ for 73.6%. NIT for 100%.

This test detected E.coli levels of 102 cfu/ml.

267 of these were positive, while 39 were negative.

Additionally, in over 300 samples, infection with other bacteria was also observed. These results indicated that the microorganism was cloned in the host.

This paper evaluated the prospective observational study in cohort study mode and chose the empirical treatment.

This was because the process was capable of detecting clonal identities and the specificity was high. The specificity was determined within hours.

Clonal diagnostics are cost-effective and reduce the need for ineffective antibiotics.

The antibiograms allow researchers to pinpoint the exact process of treatment.

Relevant questions are:* How does the phenomenon antimicrobial resistant affect the process for prescribing antibiotic medication to patients?* How does clonal diagnosis aid in the selection of empirical drugs?* What is behind the inclusions of 10%, 20%, or 30% of the threshold value for antibiotic resistance?

The use of bacterial clonal diagnostics for evidence-based empiric drug selection

PloS one 12(3), e0174132.