CLS 513 Advanced Diagnostic Microbiology

Question:

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A rapid and secure one-step extraction process for the identification and classification of Brucella species at genus level and species level using MALDI-TOF mass spectrumtry.

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A brief introduction and method to the paper is required. Then, talk about the findings and what each one means.

Answer:

Brucellosis, which is a zoonotic illness, continues to be a threat for domestic livestock in the world. This disease has caused financial losses and has been a problem for the livestock industry.

Brucellosis can be transmitted to humans by contaminated meat or dairy products. It is therefore important to develop a rapid and accurate identification method.

The current identification methods are costly and time-consuming. MALDITOF base mass is spectrometry, which has recently been developed, offers a viable alternative.

This article presents a new method for extracting protein and a reference database that can be customized.

The database contained a list of Brucella strains widely distributed.

This database was then used to compare infected cattle samples.

The assay could identify 99.5 percent of Brucella isolates at the genus level, and 97% at the species level.

The assays did not suffer from microbial isolation under different conditions.

The assay’s reliability was further confirmed by its inability to affect the protein stability of the samples while they were stored and sent out to the reference databases.

This MALDITOF assay could not deduce an accurate phylogenetic trees, which means that the protein profile did not correspond to evolution of genes.

The MALDITOF assay, which is based upon the collected data, is a reliable, precise, and fast method to identify Brucella strains.

The following questions were raised after careful review of this article.

What are the steps and factors that should be taken in order to increase their protocol?

What steps can be taken to ensure that a dendrogram can be deciphered accurately?

A sample might be shipped from Africa to a lab in 72 hours.

This assay would still be reliable and efficient if the samples were kept longer than 72 hours.